_This web page was produced as an assignment for Genetics 677, an undergraduate course at UW-Madison.
Protein-Protein Interactions
Protein-protein interactions are often thought of direct protein-protein binding; however, there are several other actions carried out by indirect protein-protein interactions such as: activation, inhibition, reaction, post-translational modification, catalysis, ect. All of these interactions create a network, or an Interactome, to carry out specific biological functions. Understanding these networks, or systems biology, is often very complex but creates the whole picture of how a specific function is carried out. Visualization of the interactions, in numerous species, are available using databases such as STRING.
The STRING database is used for numerous species, which is beneficial when researching human homologs. For homolog analysis, STRING can be used to determine whether a protein has the same function as is does in humans, or whether it interacts with the same proteins as it does in humans.
The STRING database is used for numerous species, which is beneficial when researching human homologs. For homolog analysis, STRING can be used to determine whether a protein has the same function as is does in humans, or whether it interacts with the same proteins as it does in humans.
Analysis
The interactome was created via STRING for the human HLA-C and the zebrafish HLA-C homolog (zgc:64115). The zebrafish interactome was created because it is the most distant ancestor and it is interesting to find HLA-C's most basic/ancient functions. One conserved function between the two interatomes was apoptosis. Both webs include a protein vital for functional apoptosis: IL2 for human and itm2bb for zebrafish.
Human HLA-C Interactions
The image to the left is the HLA-C interactome provided by the STRING database. This is an expanded view (more interactions) including the type of actions depicted by the colored lines: green for activation and blue for binding.
(IL2 functions in apoptosis).
(IL2 functions in apoptosis).
Zebrafish Homolog Interactions
The image to the left is the HLA-C interactome provided by the STRING database. The circle in red is zebrafish homolog, zgc:64115. Itm2bb functions in apoptosis; apoptosis is a conserved function with the human HLA-C web.
References
1. STRING
_Site created by Valeri Lapacek
Genetics 677 Assignment, Spring 2012
University of Wisconsin-Madison
Last Updated: 5/23/2012
Genetics 677 Assignment, Spring 2012
University of Wisconsin-Madison
Last Updated: 5/23/2012